| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.088821 |
| Chromosome: | chromosome 7 |
| Location: | 5952931 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g354450 | CYP743B1,CYP22,CYP743B3 | Cytochrome P450, CYP197 superfamily; (1 of 19) 1.14.14.1 - Unspecific monooxygenase / Xenobiotic monooxygenase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCACTGCAGCCAGGGGAACACGTTCAGCA |
| Internal bar code: | CAAGCCCAGCGCAATGGGTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 666 |
| LEAP-Seq percent confirming: | 98.7578 |
| LEAP-Seq n confirming: | 159 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACTTGTAGCCGATGCACA |
| Suggested primer 2: | AGATTTGGGTATCAGCACGC |