| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.088843 |
| Chromosome: | chromosome 1 |
| Location: | 875236 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g004600 | RWP12 | (1 of 16) PF02042 - RWP-RK domain (RWP-RK); RWP-RK transcription factor | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGACGGGTCCAGCCAGTGGGGCATGAAGCG |
| Internal bar code: | ATTGGGTCAACGTCCGACAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 846 |
| LEAP-Seq percent confirming: | 98.6436 |
| LEAP-Seq n confirming: | 1309 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCCAGTCCTCAACACCAT |
| Suggested primer 2: | ATGAGGATGACGAGGACGAC |