| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.088845 |
| Chromosome: | chromosome 6 |
| Location: | 31907 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g248900 | (1 of 21) IPR001611//IPR003591 - Leucine-rich repeat // Leucine-rich repeat, typical subtype | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGCCGCAAGCAGTTGCAGCAGTTCTACC |
| Internal bar code: | GGTGCGTGTTGGATCGGCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 173 |
| LEAP-Seq percent confirming: | 55.5371 |
| LEAP-Seq n confirming: | 2342 |
| LEAP-Seq n nonconfirming: | 1875 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGTCATACGACAGCTCCAG |
| Suggested primer 2: | GAAAGCCTATCCCCGCTTAC |