| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.088861 |
| Chromosome: | chromosome 12 |
| Location: | 7296234 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g557600 | ADK4 | Adenylate kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTGCAGCGACGTGGCTGCAAGAAAGGCG |
| Internal bar code: | ACATTACGGCCCAGACATGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 202 |
| LEAP-Seq percent confirming: | 97.9309 |
| LEAP-Seq n confirming: | 10602 |
| LEAP-Seq n nonconfirming: | 224 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTACAGCCGTACAGGGCAT |
| Suggested primer 2: | TAACTGTCCATGCCCTCCTC |