Insertion junction: LMJ.RY0402.088881_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.088881
Chromosome:chromosome 17
Location:7032054
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g746697 FAP249 Flagellar Associated Protein 249; (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTTCAGCACGTGTCGCGACGTGTCTGGTG
Internal bar code:AGTACTCACTTCGGAGTTACGA

Confirmation - LEAP-Seq

LEAP-Seq distance:57
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GCGCTAGCGAGCAGTAAGTT
Suggested primer 2:TATTGGTGTCCTGAGGGAGG