| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.088882 |
| Chromosome: | chromosome 16 |
| Location: | 1088030 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g649600 | PUF4 | Puf protein; (1 of 1) K14790 - nucleolar protein 9 (NOP9) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTGGATATTTACAGCGCATAATAACAAC |
| Internal bar code: | ACATCGGCCTTATGTGAGAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 543 |
| LEAP-Seq percent confirming: | 99.7236 |
| LEAP-Seq n confirming: | 1804 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTTACAAGCGTGAACAGG |
| Suggested primer 2: | CGCCGTCAGGGAAATATAGA |