| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.088909 |
| Chromosome: | chromosome 1 |
| Location: | 2683944 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g015850 | FAP68 | Flagellar Associated Protein 68 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACACACCGGTCACTGGAGTTGCGTGCGG |
| Internal bar code: | GTAAGAATCGACAACGTCGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 170 |
| LEAP-Seq percent confirming: | 15.698 |
| LEAP-Seq n confirming: | 605 |
| LEAP-Seq n nonconfirming: | 3249 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCAAAACTCCAACCCCAA |
| Suggested primer 2: | CCCTCTGTAACATCCGCATT |