| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.088948 |
| Chromosome: | chromosome 2 |
| Location: | 2651477 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTTTCTTTTCTGGCGCGCGATGGTGGTG |
| Internal bar code: | AGCTGCCCGGATCGCACGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 21 |
| LEAP-Seq percent confirming: | 40.0 |
| LEAP-Seq n confirming: | 36 |
| LEAP-Seq n nonconfirming: | 54 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACATCTGGCCCGTTTAGTT |
| Suggested primer 2: | CAAGGTTAATCAGGGGCAGA |