| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.088949 |
| Chromosome: | chromosome 15 |
| Location: | 712393 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g640600 | (1 of 1) PF00775 - Dioxygenase (Dioxygenase_C) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCCGCCCACCCCCCAAGCCGTCGCCGCG |
| Internal bar code: | TCGTACCGCAGATTCGGATTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 180 |
| LEAP-Seq percent confirming: | 98.1132 |
| LEAP-Seq n confirming: | 312 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTTATTATGTTTCGCCCA |
| Suggested primer 2: | CGGTAGGACCACAGGTAGGA |