| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.088961 |
| Chromosome: | chromosome 1 |
| Location: | 120566 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g000900 | CPLD20 | Conserved in the Plant Lineage and Diatoms; (1 of 2) PF07082 - Protein of unknown function (DUF1350) (DUF1350) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGGTCGGTGCTTGCCTTCATGCCGGGCG |
| Internal bar code: | TAGGCGCCCCGTCTGACCGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 281 |
| LEAP-Seq percent confirming: | 99.8571 |
| LEAP-Seq n confirming: | 699 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCTTGACAAGATTGTGGT |
| Suggested primer 2: | GTGGTCTTGCGTGTCCCTAT |