Insertion junction: LMJ.RY0402.088965_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.088965
Chromosome:chromosome 2
Location:6312883
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g114850 (1 of 1) PTHR22904:SF169 - RNA POLYMERASE II-ASSOCIATED PROTEIN 3 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATAGCATTGCGGGGCGAGGTGATGTTGGAG
Internal bar code:TTTGCGCTGTTACCGGAGTGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:239
LEAP-Seq percent confirming:96.5462
LEAP-Seq n confirming:1202
LEAP-Seq n nonconfirming:43
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GAAGGGTTCCGGAAGAGAAG
Suggested primer 2:GCGGCTCAACATAGGTGAAT