| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.088987 |
| Chromosome: | chromosome 8 |
| Location: | 677053 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g360100 | DUR2 | Allophanate hydrolase; (1 of 1) PTHR11895//PTHR11895:SF24 - AMIDASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCGGCTGGCAGGGGAGCAGTGTTGTGCA |
| Internal bar code: | CGGCCCATGGTTGGTGGGATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 484 |
| LEAP-Seq percent confirming: | 98.2231 |
| LEAP-Seq n confirming: | 2377 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTAAACGCATCCCGTTCAG |
| Suggested primer 2: | ATGTGCATGTGTGTGTGTCG |