| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.089000 |
| Chromosome: | chromosome 5 |
| Location: | 872285 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g247650 | (1 of 32) IPR000536 - Nuclear hormone receptor, ligand-binding domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTGTTTGGTTTGCTTTAACGTGATGGCA |
| Internal bar code: | GCAATGCGGCTTGGTATCCGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 137 |
| LEAP-Seq percent confirming: | 35.6436 |
| LEAP-Seq n confirming: | 1908 |
| LEAP-Seq n nonconfirming: | 3445 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTCTGTCTTTCAATCCCA |
| Suggested primer 2: | GTACGGCTGATACGGGTTGT |