| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.089028 |
| Chromosome: | chromosome 1 |
| Location: | 5475878 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g038550 | SQD2 | Sulfoquinovosyldiacylglycerol synthase; (1 of 2) K06119 - sulfoquinovosyltransferase (SQD2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGTGGCGGGTCTCGGGCTGGTACCCACG |
| Internal bar code: | CCCCGTGATGGCCTGAGAATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 50 |
| LEAP-Seq percent confirming: | 90.7453 |
| LEAP-Seq n confirming: | 5187 |
| LEAP-Seq n nonconfirming: | 529 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACTACGAAGCCCAGCGTCT |
| Suggested primer 2: | GGTTGTAGCTACCAGCCCAA |