| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.089068 |
| Chromosome: | chromosome 10 |
| Location: | 1874860 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g431550 | ADH6 | Alcohol dehydrogenase; (1 of 1) PTHR11695//PTHR11695:SF559 - ALCOHOL DEHYDROGENASE RELATED // GALACTITOL-1-PHOSPHATE 5-DEHYDROGENASE | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACATGCAAGTACTGCTTGCAGCCTGGGA |
| Internal bar code: | TGGGGTGCCGGATAAGGCGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 729 |
| LEAP-Seq percent confirming: | 99.7595 |
| LEAP-Seq n confirming: | 3733 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCTCACTGATGTGTGTTG |
| Suggested primer 2: | TCCAAAAGGTCGATGCCTAC |