| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.089086 |
| Chromosome: | chromosome 13 |
| Location: | 838858 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g567300 | (1 of 1) PTHR18968//PTHR18968:SF86 - THIAMINE PYROPHOSPHATE ENZYMES // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTGTGTGGGGCGGTGTGGGGTTGTGTGC |
| Internal bar code: | GGTCCGGGGTGTTCGTACGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 314 |
| LEAP-Seq percent confirming: | 75.0168 |
| LEAP-Seq n confirming: | 1117 |
| LEAP-Seq n nonconfirming: | 372 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTGGGTTGAAATGCGTAG |
| Suggested primer 2: | CACTGGCACTGCTGGTAATG |