Insertion junction: LMJ.RY0402.089104_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.089104
Chromosome:chromosome 6
Location:3446876
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre06.g278093 ELG20 Exostosin-like glycosyltransferase 20; (1 of 34) 2.4.2.41 - Xylogalacturonan beta-1,3-xylosyltransferase / Xylogalacturonan xylosyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGGTCTGAGCCTAGGTGACGTACCCGCCC
Internal bar code:CGGTCCACGGTGGAAGTCGCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:221
LEAP-Seq percent confirming:81.4925
LEAP-Seq n confirming:1638
LEAP-Seq n nonconfirming:372
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCACGCAGCAGTAGTAGC
Suggested primer 2:CAGCACCCCCACCTAAACTA