Insertion junction: LMJ.RY0402.089128_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.089128
Chromosome:chromosome 17
Location:2695071
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre17.g717900 PHC1 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 1 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGCCAGTCAGGTAGGCGGGGCAGCTGCCC
Internal bar code:AGGTAGCACCTAGCAAATGTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:280
LEAP-Seq percent confirming:42.4547
LEAP-Seq n confirming:1055
LEAP-Seq n nonconfirming:1430
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AAGTCGTTCAGGGTGGTGTC
Suggested primer 2:TTTGACAGCCAGAACGACTG