Insertion junction: LMJ.RY0402.089152_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.089152
Chromosome:chromosome 17
Location:6208440
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g741950 BBS1 Bardet-Biedl syndrome-1 associated protein; (1 of 1) K16746 - Bardet-Biedl syndrome 1 protein (BBS1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCAACTTCGGGAGCGCTGTAAATGCGAAT
Internal bar code:GGTTTCGTAACGGCCGCGCGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:651
LEAP-Seq percent confirming:99.0963
LEAP-Seq n confirming:4496
LEAP-Seq n nonconfirming:41
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:CACCAGGTGATCGTTGTCAC
Suggested primer 2:GTCGTTTTGTCCCACTCGAT