| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.089158 |
| Chromosome: | chromosome 16 |
| Location: | 4667163 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g687350 | ACO3,ACX3 | (1 of 1) PTHR10909:SF11 - ACYL-COENZYME A OXIDASE-LIKE PROTEIN; Acyl-CoA oxidase/dehydrogenase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGACAAGTGGAAGCTCTTCGCCGGAAAG |
| Internal bar code: | TGGTCCGGAGGTATTCCCGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 33 |
| LEAP-Seq percent confirming: | 99.3151 |
| LEAP-Seq n confirming: | 145 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTTCCCAGTCCCATCACT |
| Suggested primer 2: | GGGGTAAGCTCTGGGGTAAG |