| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.089159 |
| Chromosome: | chromosome 2 |
| Location: | 7122254 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g146650 | TTL3,SSA11 | (1 of 2) K16582 - tubulin polyglutamylase TTLL6/13 [EC:6.-.-.-] (TTLL6_13); Tubulin tyrosine ligase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGCAAATGTCGTGTCATCCTAGCCAACA |
| Internal bar code: | TTTTCGGACATTGTCTCCAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 631 |
| LEAP-Seq percent confirming: | 99.6124 |
| LEAP-Seq n confirming: | 2827 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATTATCATGCCTCGGAGA |
| Suggested primer 2: | ATAAGCTGCCACCCATGAAC |