| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.089162 |
| Chromosome: | chromosome 17 |
| Location: | 5487154 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g737351 | 3'UTR/CDS|intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCTGAGGCCGGTCTGCTAGGGCCTACGC |
| Internal bar code: | AACCCGCGTAAGGTAACGGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 418 |
| LEAP-Seq percent confirming: | 98.5849 |
| LEAP-Seq n confirming: | 1045 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGGAGGTGTAGGTAGAGG |
| Suggested primer 2: | CAATGACAACGTCAACCGAC |