| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.089193 |
| Chromosome: | chromosome 8 |
| Location: | 1720516 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g365950 | (1 of 3) PTHR22753:SF1 - TRANSMEMBRANE PROTEIN 68 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACTCACCGACATGTCAAATGCGTACAGT |
| Internal bar code: | ACTTTGGCCCCGCCTGCGAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 148 |
| LEAP-Seq percent confirming: | 21.2413 |
| LEAP-Seq n confirming: | 794 |
| LEAP-Seq n nonconfirming: | 2944 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTCTCACCACCACATCTT |
| Suggested primer 2: | TTACCTCCTCCCCAATCTCC |