| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.089197 |
| Chromosome: | chromosome 11 |
| Location: | 3686610 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g482626 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATATGCCGCTGTAATGCAAGCATACGTG |
| Internal bar code: | GCATATACGTGCAGCACGGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 423 |
| LEAP-Seq percent confirming: | 99.6029 |
| LEAP-Seq n confirming: | 13545 |
| LEAP-Seq n nonconfirming: | 54 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACACATTCCATGGCTGAA |
| Suggested primer 2: | TTCCTGACATCCCGTCTACC |