| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.089197 |
| Chromosome: | chromosome 16 |
| Location: | 2853016 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g663500 | RPN10 | (1 of 1) K03029 - 26S proteasome regulatory subunit N10 (PSMD4, RPN10); 26S proteasome regulatory subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGGTGTGCGGTACAGAGTGGCAAAATCG |
| Internal bar code: | TGGAGGTTTCGGGGTGTACAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 297 |
| LEAP-Seq percent confirming: | 94.1341 |
| LEAP-Seq n confirming: | 1011 |
| LEAP-Seq n nonconfirming: | 63 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCGCTCAGCCTAAAACTGT |
| Suggested primer 2: | GCACTCTACGCACACCTTGA |