| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.089215 |
| Chromosome: | chromosome 10 |
| Location: | 4287983 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g450650 | (1 of 34) PF00168 - C2 domain (C2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGCTGGCAGGCTGCAACCTCTGGATCC |
| Internal bar code: | TATTCAATCCCGGTACGTACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 15 |
| LEAP-Seq percent confirming: | 75.8621 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCCTGGTGTAAGTCCGTG |
| Suggested primer 2: | AGGCAAGCAGACTGAGGTGT |