Insertion junction: LMJ.RY0402.089232_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.089232
Chromosome:chromosome 2
Location:7042115
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g147450 (1 of 5) K14347 - solute carrier family 10 (sodium/bile acid cotransporter), member 7 (SLC10A7, P7) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCAGCTAGCGGTTGCCATGGGCATCAACG
Internal bar code:TCACAGAGTTTAGGCTGCAAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:332
LEAP-Seq percent confirming:78.586
LEAP-Seq n confirming:5769
LEAP-Seq n nonconfirming:1572
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GTGCTCTTGACCAGGTAGGC
Suggested primer 2:TCTTGTTCCTGGAGATTCCG