| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.089241 |
| Chromosome: | chromosome 10 |
| Location: | 3852616 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g447500 | KIN12-3,KIN16B,KIN16-2 | Kinesin motor protein; (1 of 1) K10392 - kinesin family member 1 (KIF1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGGAGGTGCGCGCGTAGGCGGCCCGCAG |
| Internal bar code: | ATGTGCTGGGGGACGCGCACCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 588 |
| LEAP-Seq percent confirming: | 92.539 |
| LEAP-Seq n confirming: | 5048 |
| LEAP-Seq n nonconfirming: | 407 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGCATTCTCCCTGCACAC |
| Suggested primer 2: | AAGATTACCAACCAGGTGCG |