Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.089276 |
Chromosome: | chromosome 10 |
Location: | 2806866 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre10.g439250 | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGAACACCTTCTCTCCTCCACCTGCAAGC |
Internal bar code: | CTGGTTAACCAGCCGCGGGTGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 771 |
LEAP-Seq percent confirming: | 98.0325 |
LEAP-Seq n confirming: | 4883 |
LEAP-Seq n nonconfirming: | 98 |
LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTGCCTTCAAGATCTCTGGC |
Suggested primer 2: | GGTATGTCGGCACCAGAAGT |