Insertion junction: LMJ.RY0402.089282_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.089282
Chromosome:chromosome 16
Location:1900470
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g655950 TRP2 Transient receptor potential ion channel protein; (1 of 3) IPR002110//IPR005821//IPR020683 - Ankyrin repeat // Ion transport domain // Ankyrin repeat-containing domain CDS|outside_mRNA

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCGAGTTTCGCCTGTCCCGTCCGAGCTGC
Internal bar code:CCTGTACGATCTCACGCCGTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:285
LEAP-Seq percent confirming:97.7099
LEAP-Seq n confirming:768
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CCAGGTTCTCAAGTTCTCGC
Suggested primer 2:CGTATGTGACGTCGTGGTTC