| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.089303 |
| Chromosome: | chromosome 8 |
| Location: | 4833159 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g384500 | (1 of 1) PF08856 - Protein of unknown function (DUF1826) (DUF1826) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGGGGCCTGGGAGGAGGGGAGGGTGAGG |
| Internal bar code: | CCATACAATCTATATTTCATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 262 |
| LEAP-Seq percent confirming: | 99.5556 |
| LEAP-Seq n confirming: | 1120 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAAACACCATCAGCACCAC |
| Suggested primer 2: | ATCAGATGACACCACGACCA |