Insertion junction: LMJ.RY0402.089322_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.089322
Chromosome:chromosome 6
Location:7018778
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre06.g296850 FAP81,DLEC1 (1 of 8) IPR008962 - PapD-like; Flagellar Associated Protein 81 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACTCCGAGGCGCAGAAGGTGACGGTGAAC
Internal bar code:CCTGCGAGTGGCCGGCGCTGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:72
LEAP-Seq percent confirming:99.8415
LEAP-Seq n confirming:630
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CCGACTGAGTTCTTTCCTGC
Suggested primer 2:CGTGGGTTAGTGGGTATGCT