| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.089340 |
| Chromosome: | chromosome 11 |
| Location: | 1926213 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g468350 | (1 of 1) K00486 - kynurenine 3-monooxygenase (KMO) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCGGTGTATCCACATCCCGGGTCTCGGT |
| Internal bar code: | AGGAGACGACTAGTGCCCCACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 658 |
| LEAP-Seq percent confirming: | 99.2017 |
| LEAP-Seq n confirming: | 3355 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTACAACCCTACCTCCCCC |
| Suggested primer 2: | GCAACCTAACTACCCGACCA |