| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.089356 |
| Chromosome: | chromosome 8 |
| Location: | 1125172 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g362600 | BLA1 | (1 of 2) PF00144 - Beta-lactamase (Beta-lactamase); beta-lactamase family protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCATCTGCAAGACGTTCGAATGCACGAA |
| Internal bar code: | AAAGAGACGTTCCTCTCTTATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1205 |
| LEAP-Seq percent confirming: | 99.2005 |
| LEAP-Seq n confirming: | 6948 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGCTCTGTTTCCTCCAAC |
| Suggested primer 2: | GTCACACGCATGCATAAACC |