| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.089356 |
| Chromosome: | chromosome_10 |
| Location: | 1651672 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g429750 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GAGCAGCGGCGGCCCCGGCGGTAACGGCTC |
| Internal bar code: | GGCATTATCTGGATTTTGACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 168 |
| LEAP-Seq percent confirming: | 90.1919 |
| LEAP-Seq n confirming: | 423 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCTACCACCTGTCTGCAA |
| Suggested primer 2: | AAGGAGAAGGGGAACAAGGA |