| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.089365 |
| Chromosome: | chromosome 16 |
| Location: | 2847201 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g663450 | LCI11B,BST3,LCI11 | Bestrophin 3; (1 of 10) PF01062 - Bestrophin, RFP-TM, chloride channel (Bestrophin) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGAACGTGCGCATCTCGCCCCGGTAGAA |
| Internal bar code: | AAATGGTCACTACTAGATGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 441 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 36 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCTAGTATTCATGGCCGC |
| Suggested primer 2: | CTTCGGGTGAGTCTAGGCAG |