| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.089366 |
| Chromosome: | chromosome 13 |
| Location: | 3162273 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g585250 | (1 of 1) PF08005 - PHR domain (PHR) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGGTGCCCTTCTCGCCGCACGCCGCGAC |
| Internal bar code: | ATGTGCAATTGAGAGATGATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1056 |
| LEAP-Seq percent confirming: | 99.7403 |
| LEAP-Seq n confirming: | 24583 |
| LEAP-Seq n nonconfirming: | 64 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGAGACACTTATGGTGGGT |
| Suggested primer 2: | GAGCGAGCCTGACTTGAAAC |