Insertion junction: LMJ.RY0402.089380_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.089380
Chromosome:chromosome 6
Location:4970158
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g279750 MAP1A (1 of 1) PTHR10804//PTHR10804:SF13 - PROTEASE FAMILY M24 METHIONYL AMINOPEPTIDASE, AMINOPEPTIDASE P // METHIONINE AMINOPEPTIDASE 1; Methionine aminopeptidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TATGGCAAGTAGGTATAGGGCTAACCATCC
Internal bar code:TCATCTCTCGACCGGGGCGCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:346
LEAP-Seq percent confirming:97.7605
LEAP-Seq n confirming:6286
LEAP-Seq n nonconfirming:144
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:ACTCACTGGGTCCCTTGTTG
Suggested primer 2:ACAACTCCCACCTTGCTCAC