Insertion junction: LMJ.RY0402.089395_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.089395
Chromosome:chromosome 10
Location:5220930
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g456950 CEP10 Cysteine endopeptidase; (1 of 1) IPR000668//IPR008979//IPR013128//IPR018392//IPR029062 - Peptidase C1A, papain C-terminal // Galactose-binding domain-like // Peptidase C1A // LysM domain // Class I glutamine amidotransferase-like CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCAATATCCAAGAGCGAACGATGAACTGG
Internal bar code:TCGCAGCCGTTGTTTTTCTGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:236
LEAP-Seq percent confirming:99.076
LEAP-Seq n confirming:965
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTCCTCGACCTGTATAGC
Suggested primer 2:ACGACAGCTGACAGCACAAC