Insertion junction: LMJ.RY0402.089406_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.089406
Chromosome:chromosome 14
Location:1996680
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre14.g621450 RPL5 Ribosomal protein L5, component of cytosolic 80S ribosome and 60 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTTGTGCATGCACATACACGCATAGCAAA
Internal bar code:GGCTGTTCTAGGGCAAATGCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:460
LEAP-Seq percent confirming:92.291
LEAP-Seq n confirming:850
LEAP-Seq n nonconfirming:71
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GTACATGAAGCACTTCGCCA
Suggested primer 2:CACAAGACACCACGAGAGGA