| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.089412 |
| Chromosome: | chromosome 14 |
| Location: | 3099341 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g628650 | MME4 | NADP-dependent malic enzyme 4; (1 of 5) 1.1.1.40 - Malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+)) / Pyruvic-malic carboxylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAATCGGCACCCGGTGGGCGGCACGTAA |
| Internal bar code: | ATGTTACGCAGAGCTTGTATAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 645 |
| LEAP-Seq percent confirming: | 95.6522 |
| LEAP-Seq n confirming: | 198 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAATGTGTGGGAGCGGTACA |
| Suggested primer 2: | TTCGCCTTACTTCCTAGCCA |