| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.089441 |
| Chromosome: | chromosome 3 |
| Location: | 6570227 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g195950 | (1 of 7) PTHR11584:SF381 - PROTEIN PQN-80, ISOFORM B | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGGAGCACGGGCGCGCTGCGCAGGTGAG |
| Internal bar code: | TCTCTCACCGTTCTCTCAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 602 |
| LEAP-Seq percent confirming: | 97.9863 |
| LEAP-Seq n confirming: | 5450 |
| LEAP-Seq n nonconfirming: | 112 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATCGAGGACATGCGATAGG |
| Suggested primer 2: | GTAAGGATGCGGACTGAAGG |