| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.089456 |
| Chromosome: | chromosome 1 |
| Location: | 4583642 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g031350 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGGCCATTTGCTGCTGCTGCGGCGGCTT |
| Internal bar code: | CAGTGTGCTGGAACCCAGCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 192 |
| LEAP-Seq percent confirming: | 94.448 |
| LEAP-Seq n confirming: | 1463 |
| LEAP-Seq n nonconfirming: | 86 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGTCGGTTGTGGACTCAC |
| Suggested primer 2: | CAAGATAGCGGGCAAAGAAG |