| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.089464 |
| Chromosome: | chromosome 17 |
| Location: | 4429234 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g731850 | SDR29 | Short-chain dehydrogenase/reductase; (1 of 1) 1.3.1.34 - 2,4-dienoyl-CoA reductase (NADPH) / 4-enoyl-CoA reductase (NADPH) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTATCGGACGGCCTTGATGCAAGCGCTGC |
| Internal bar code: | TAGCTTCGTGGCCACTGTACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1484 |
| LEAP-Seq percent confirming: | 99.2916 |
| LEAP-Seq n confirming: | 1682 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGAAGGAGTACCGCTATTG |
| Suggested primer 2: | TAAACCCAACCCAATGGAAA |