Insertion junction: LMJ.RY0402.089465_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.089465
Chromosome:chromosome 12
Location:5651449
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g532350 CAV8 (1 of 2) PF00520//PF08016 - Ion transport protein (Ion_trans) // Polycystin cation channel (PKD_channel); Voltage-gated Ca2+ channel, alpha subunit intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGTCACCGGCACCGCACCGCACGGACCGC
Internal bar code:CAAGATTAAACGGACCACCGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:108
LEAP-Seq percent confirming:97.1163
LEAP-Seq n confirming:2930
LEAP-Seq n nonconfirming:87
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:CTTTGCACCACTGTTCTCCA
Suggested primer 2:GTTGCTGGTCTTGAGGAAGC