| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.089469 |
| Chromosome: | chromosome 9 |
| Location: | 2418153 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g391600 | MEP1 | Putative IgA-specific metalloendopeptidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGCTGCCGGCTTCACTGCAAGCGCCATC |
| Internal bar code: | TAGTGCACTATATCCCCGTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 721 |
| LEAP-Seq percent confirming: | 97.3469 |
| LEAP-Seq n confirming: | 2385 |
| LEAP-Seq n nonconfirming: | 65 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACTTGTGACACCTGCTGC |
| Suggested primer 2: | COULD_NOT_FIND_PRIMER |