Insertion junction: LMJ.RY0402.089475_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.089475
Chromosome:chromosome 3
Location:5548882
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre03.g186350 (1 of 3) PF03803 - Scramblase (Scramblase) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGCGCGCGCAGGTTCAGGCCGCCAAACGC
Internal bar code:CGTGCGGGACGGTGCCGTCCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:245
LEAP-Seq percent confirming:99.4907
LEAP-Seq n confirming:1172
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CGGACACCTACCGTACCAGT
Suggested primer 2:AGGCAGCCAACAAGTACCAC