| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.089506 |
| Chromosome: | chromosome 13 |
| Location: | 805279 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g567100 | GLDH1,GLDH,ALO1 | (1 of 1) 1.3.2.3 - L-galactonolactone dehydrogenase / L-galactono-gamma-lactone dehydrogenase; L-galactono-1%252C4-lactone dehydrogenase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTACCTGCCGCCCGAGCCGGAGAAGAGGG |
| Internal bar code: | GCAGAGTGGCCAGGGCACGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 47 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCAGGAACTGTGTATCGT |
| Suggested primer 2: | CGCACCTAGTAAGCCACACA |