| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.089510 |
| Chromosome: | chromosome 10 |
| Location: | 6129086 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g463750 | CYG27 | Adenylate/guanylate cyclase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGCGCTGCCGTCCAACTTCAATGAGCGT |
| Internal bar code: | AAAACTACGACTTGTTACTTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 861 |
| LEAP-Seq percent confirming: | 99.5028 |
| LEAP-Seq n confirming: | 2802 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGATGGGGAAGAGGTGTGT |
| Suggested primer 2: | TACTTGGTGCACATGCCATT |