| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.089516 |
| Chromosome: | chromosome 6 |
| Location: | 2973401 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g273300 | TST1 | (1 of 2) 6.1.1.3 - Threonine--tRNA ligase / Threonyl-tRNA synthetase; Threonyl-tRNA synthetase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCATGACTCATTCGTGCATGACTGCAAC |
| Internal bar code: | GGTTCGGACATTATCACTCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 290 |
| LEAP-Seq percent confirming: | 99.7932 |
| LEAP-Seq n confirming: | 1930 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAAGAGCGGCTCAGAAGAG |
| Suggested primer 2: | CATTCCAATCCCAAATGGAC |